Collagen Coated 6 Well Plate

The grade of jellagen jellyfish collagen used to coat this.

Collagen coated 6 well plate.

In vitro use of collagen can exert effects on the adherence morphology growth migration and differentiation of a variety of cell types. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. Collagen is the most widely used extracellular matrix ecm protein for cell culture facilitating cell attachment growth differentiation migration and tissue morphogenesis. Gibco collagen i rat tail is now available in pre coated tissue culture plates to help save time at the bench.

Corning biocoat collagen i 6 well clear multiwell plate is comprised of clear tc treated polystyrene with a uniform application of rat tail collagen type i. Flat bottom 5 pack 50 case 389 83 per case. To coat culture wells we dilute 20μl of collagen stock in 1ml of 30 ethanol per well 6 well plates and. 266 µl of naoh 0 1 m.

Combine 5 6 ml of 10x pbs with 194 4 ml of 1x dpbs. Procedure for one 6 well plate or 12 well plate under the culture hood prepare 200 ml of 1 25x dpbs. Collagen i found in most tissues and organs is most plentiful in dermis tendon and bone. Under the culture hood prepare a collagen coating solution 300 μg ml by combining the following into a 50 ml centrifuge tube or similar sterile container in order.

Ready to use room temperature storage. We use 2 5mg ml collagen from rat tail sigma stock solution in 0 2 sterile acetic. 24 and 96 well cultureware plates coated with jellyfish collagen suitable for cell culture research purposes.